tion in biomass ? Limitation of plant production by nitrogen ? Low resveratrol, resveratrol derivatives and emodin production. The efficiency of nitrogen fixation was substantially correlated with all the ratio of resveratrol to resveratrol glucoside. This indicates that knotweed CAL-101 contributed towards the energy cost of nitrogen fixation for melilot and that there's an exchange of organic substances in between these two plant species. There appeared to be differences in between the substrates. Compost was revealed to have a low efficiency of N fixation and, at the same time, showed a greater proportion of resveratrol glucosides compared with its aglycones. The opposite was true for the clayish low nutrient substrates, clay and loess.
Clay of miocene origin was obtained from spoil banks that were produced up with the same material CAL-101 as the soil within the field experiment , loess from nearby loess deposits and compost was that utilised for dump reclamation. The chemical composition with the substrates is shown in Table 2. Ten pots were filled with 7.25 kg of clay each and 2 l of certainly one of the following substrates: loess ; compost , composed of a 1:1 mixture of prevalent compost and a cellulose rich paper mill by product known as Lignocel ; or clay enriched having a slowrelease biofertilizer Conavit? ; or clay enriched with Conavit and 50 ml of arbuscularmycorrhizal product Symbivit? . For technical sheet and composition of both merchandise see http: www. symbiom.cz. A mixture of six mycorrhizal fungi species with at the very least 80,000 living propagules per litre in zeolit or spongilit was added to each pot, in addition to expanded clay enriched with all-natural fertilizer.
Conavit can be a completely all-natural slow nutrient releasing fertilizer composed of sea algae, humus substances, ground minerals and rocks, and can be a all-natural source of keratin. A quantity of Conavit corresponding Gefitinib to 160 kg ha was applied. Symbivit was added towards the Conavit treated pots on leading with the bottom clay layer. The bottom layer of clay had a texture of larger lumps, while the overlying material was broken up into smaller particles. Twenty pots of each variant were prepared for a total of 100 pots. The pots were thoroughly wetted and kept within the greenhouse at 18 27 C. During the summer time, the whole set was transferred outdoors towards the experimental garden and was kept moist using automatic drop irrigation as needed.
Plants At the begin with the experiment, November VEGF 18, 2005, segments of R. bohemica rhizomes that had been pre cultivated in peat were very carefully prepared. Each pot received a segment of washed rhizome having a known fresh weight and a known number of buds. The average fresh weight of a segment was 3.3 g and also the average bud number was 1.6. The bud numbers did not differ substantially in between the variants. Around 40 additional segments of these rhizomes were each inserted into a small pot of perlite in an effort to generate plantlets in case a number of the plants within the experimental pots failed to grow. This proved to be a terrific advantage mainly because a number of the rhizomes, especially those from the variant grown with Conavit, did not generate any plantlets. This can be most likely because of the adverse effect of humic substances on the growth of fine roots.
The dormant rhizomes were later exchanged for mature plantlets from the perlite pots. The pre grown plantlets continued their growth with no restriction, regardless of which variety of substrate they were transplanted into. Immediately after three months, the R. bohemica plants were well established and white melilot seeds Gefitinib were added to 10 out with the 20 pots of each variant. The capability with the seeds to germinate was assessed prior to seeding and was discovered to be 57 based on the average from 10 Petri dishes, each with 25 seeds. You will find roughly 500 seeds in one gram. Immediately after the first season, the plants were harvested in September 2006. We measured CAL-101 twig numbers, lengths and dry masses of both Reynoutria and Mellilotus, and excised 100 mm segments with the new rhizomes, which formed alongside the pot wall, for chemical analyses.
The ramification with the branches was also taken into account; the lengths of all of the key branches Gefitinib rising from the soil, as well as the lengths of all of the side branches, were measured and evaluated. Fine roots were sampled, while knotweed roots were hand separated from the melilot roots, and both were stained and inspected for the presence of mycorrhiza. The experiment was terminated right after the second season in September 2007. At the end with the experiment, both the aboveground and belowground biomass were measured, the fine roots were sampled for mycorrhiza and larger roots and rhizomes were thoroughly washed using air and water pressure. These were then dried and ground for analysis. Melilot was allowed to grow with no restriction throughout the first season, but plants were repeatedly cut throughout the second season to maintain a height of 30 cm. Field experiment The centre with the 1 ha experimental non irrigated field is at a location of 50 35’N, 13
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